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MULTIMODE MICROPLATE READER
  • 产品概述
  • 产品参数
  • 产品特性
  • 产品应用

Spark multi-functional microplate detection platform can provide customized solutions for almost all drug development and frontier life science research. Researchers can configure the instrument function modules on this platform according to the current needs, and upgrade to acquire other technologies and characteristics needed in the future.

Spark multicolor chemiluminescence module also has unparalleled flexibility to meet the detection needs of all chemiluminescence experiments, including flash, glow, BRET and laser-based Alpha technology.

Spark uses a dedicated high-speed grating for optical absorption measurements, with unparalleled wavelength accuracy. Combining the vertical colorimetric module with the proprietary NanoQuantPlate, Spark provides an integrated solution for ELISA, microDNA/protein quantification and fast spectral scanning experiments.

Spark is equipped with open-field cell imaging function, combined with its similar incubator environment control, can carry out long-term cytological experiments and online monitoring of cell growth. Conditional automation workflow can minimize the manual operation time and increase the reliability of the experiment, and realize the long-term dynamic experiment of precious plants.

Standard form

Light source: special flashing xenon lamp

Wavelength range: EX: 230-900 NM

EX:280-900nm

Wavelength accuracy: Ex:<1nm; Em:<2nm

Wavelength repeatability: < 1nm

Bandwidth: Fixed @20nm

Spectroscope: built-in 50%, 510 nm dichroic mirror

Hole Scanning: Up to 100x100 Data Points

Fluorescence intensity (FI) detection limit

Filter - Top: <25amol/well (100ul; 384well)

Fusion-Top: <35amol/well(100ul; 384well)

Grating-top: < 50 amol / well (100ul; 384 well)

Filter - bottom: <500 amol/well (200ul; 96well)

Fusion - Bottom: <700amol/well (200ul; 96well)

Grating-bottom: < 800amol/well (200ul; 96well)

Fluorescence polarization (FP)

Wavelength range: 300-850 NM

Accuracy - Filter: <1.5mP

Accuracy-Fusion: <2.5mP

Accuracy-grating: <3.0mP

Time-resolved fluorescence (TRF)

Detection limit-filter: < 4.0amol/well (100ul; 384well)

Detection limit-fusion: < 6.5 amol / well (100ul; 384 well)

Detection limit-grating: < 10amol/well (100ul; 384well)

Fastest read time

96 orifice plate (FI) = 13sec

384 orifice plate (FI) < 30sec

Basic detection mode

Optical Absorption - Including Ultraviolet/Visible Spectral Scanning

Fluorescence Top and Bottom Reading - Including Fluorescence Spectroscopy Scanning (3D)

Time-resolved fluorescence (TRF) - including TRF spectral scanning

Fluorescence Resonance Energy Transfer (FRET)

Time-resolved fluorescence resonance energy transfer (TR-FRET)

Fluorescence polarization (FP)

Chemiluminescence, Glow, Flash, Multicolor Luminescence and High Sensitivity Luminescence Spectroscopy Scanning

AlphaScreen <, AlphaLISA <, and AlphahaPlex <.

Automated real-time bright-field imaging of cells-cell counting and cell confluence

Additional functions

Light absorption vertical colorimetric cup

NanoQuant Microplate

Temperature control (room temperature + 3 -42)

Automatic liquid sampler with heating and stirring

CO 2 & O 2 gas control module

Volatilization protection (humidity box)

Te-Cool () Refrigeration (Active Temperature Control Range 18-42 C)

Built-in automatic capping

QC tools (IQ/OQ services)

Integral Spark-Stack Microporous Plate Stack

Application:

*ELISA

*Quantitative analysis of Microamounts of DNA/RNA

*Nucleic acid labeling efficiency

*Quantification of protein

*Reporting genes

*Homogeneous time-resolved fluorescence of HTRF

*Transcreener

*DLR Double Reporter Gene

*BRET - Including NanoBRET

*Cell count and viability

*Assessment of Convergence Degree

*Cell migration and healing

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